Journal: The Journal of Experimental Medicine

Article Title: A network of immune and microbial modifications underlies viral persistence in the gastrointestinal tract

doi: 10.1084/jem.20191473

Figure Lengend Snippet: The GIT is a long-lived reservoir of LCMV-Cl13 infection. (A and B) Mice were infected with LCMV-Arm (acute) or LCMV-Cl13 (chronic), and 5 and 8 dpi, PFU of virus per gram of tissue or per milliliter of blood plasma was determined. n = 4–5 mice per group from one experiment. *, P < 0.05 by ANOVA test. (B) H&E staining was performed on LI tissue from naive mice or 8 dpi after acute LCMV-Arm or chronic LCMV-Cl13 infection, showing a lack of epithelial or villus pathology during infection. Representative pictures from n = 5 mice per group from one experiment. Scale bars = 50 µm. (C and D) Mice were infected with LCMV-Arm or LCMV-Cl13, and 0, 8, 35, and 100 dpi, the SI, LI, and spleen were analyzed by CyTOF. Graphs show total number of (C) CD45 + viable cells and (D) the indicated immune cell subsets: CD8αβT cells (TCRβ + CD8α + CD8β + ), CD8ααT cells (TCRβ + CD8α + CD8β − ), monocytes/macrophages (CD11b + SiglecF − TCR − B220-CD11c − ), polymorphonuclear cells (CD11b + Ly6G + TCR − B220 − CD11c − ), DCs (CD11c hi MHC-II hi TCR − B220 − ), CD4 T cells (TCRβ + CD4 + ), B cells (B220 + MHC-II + ), ILCs (lin − Thy1.2 + ), and γδT cells (TCRγδ + TCRβ − B220 − ) in the SI, LI, and spleen. *, P < 0.05 (Mann-Whitney test of log-transformed data). n = 10 mice per group. Error bars indicate SEM (A–D).

Article Snippet: The samples were then washed with PBS and pulsed with 12.5 μM cisplatin in PBS for 1 min at RT before quenching with CyTOF staining media (Mg + /Ca 2+ HBSS containing 2% FBS [Multicell], 10 mM Hepes [Corning]) and FBS underlay.

Techniques: Infection, Virus, Clinical Proteomics, Staining, MANN-WHITNEY, Transformation Assay